Laboratory Diagnosis of Emerging Human Coronavirus Infections - The State of the Art.
Identifieur interne : 000623 ( an2020/Analysis ); précédent : 000622; suivant : 000624Laboratory Diagnosis of Emerging Human Coronavirus Infections - The State of the Art.
Auteurs : Michael J. Loeffelholz [Oman] ; Yi-Wei Tang [Oman]Source :
- Emerging microbes & infections [ 2222-1751 ] ; 2020.
Abstract
The three unprecedented outbreaks of emerging human coronavirus (HCoV) infections at the beginning of 21st century have highlighted the necessity for readily available, accurate and fast diagnostic testing methods. The laboratory diagnostic methods for human coronavirus infections have evolved substantially, with the development of novel assays as well as the availability of updated tests for emerging ones. Newer laboratory methods are fast, highly sensitive and specific, and are gradually replacing the conventional gold standards. This presentation reviews the current laboratory methods available for testing coronaviruses by focusing on the coronavirus disease 2019 (COVID-19) outbreak going on in Wuhan. Viral pneumonias typically do not result in the production of purulent sputum. Thus, a nasopharyngeal swab is usually the collection method used to obtain a specimen for testing. Nasopharyngeal specimens may miss some infections; a deeper specimen may need to be obtained by bronchoscopy. Alternatively, repeated testing can be used because over time, the likelihood of the SARS-CoV-2 being present in the nasal-pharynx increases. Several integrated, random-access, point-of-care molecular devices are currently under development for fast and accurate diagnosis of SARS-CoV-2 infections. These assays are simple, fast and safe and can be used in the local hospitals and clinics bearing the burden of identifying and treating patients.
DOI: 10.1080/22221751.2020.1745095
PubMed: 32196430
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000228
- to stream PubMed, to step Curation: 000228
- to stream PubMed, to step Checkpoint: 000580
- to stream Ncbi, to step Merge: 001376
- to stream Ncbi, to step Curation: 001376
- to stream Ncbi, to step Checkpoint: 001376
- to stream Main, to step Merge: 000625
- to stream Main, to step Curation: 000623
- to stream Main, to step Exploration: 000623
- to stream an2020, to step Extraction: 000623
Links to Exploration step
pubmed:32196430Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Laboratory Diagnosis of Emerging Human Coronavirus Infections - The State of the Art.</title><author><name sortKey="Loeffelholz, Michael J" sort="Loeffelholz, Michael J" uniqKey="Loeffelholz M" first="Michael J" last="Loeffelholz">Michael J. Loeffelholz</name><affiliation wicri:level="1"><nlm:affiliation>Cepheid, Sunnyvale, California, USA, michael.loeffelholz@cepheid.com.</nlm:affiliation><country wicri:rule="url">Oman</country><wicri:regionArea>Cepheid, Sunnyvale, California, USA</wicri:regionArea><wicri:noRegion>USA</wicri:noRegion></affiliation></author><author><name sortKey="Tang, Yi Wei" sort="Tang, Yi Wei" uniqKey="Tang Y" first="Yi-Wei" last="Tang">Yi-Wei Tang</name><affiliation wicri:level="1"><nlm:affiliation>Cepheid, Danaher Diagnostic Platform, Shanghai, China, yi-wei.tang@cepheid.com.</nlm:affiliation><country wicri:rule="url">Oman</country><wicri:regionArea>Cepheid, Danaher Diagnostic Platform, Shanghai, China</wicri:regionArea><wicri:noRegion>China</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32196430</idno><idno type="pmid">32196430</idno><idno type="doi">10.1080/22221751.2020.1745095</idno><idno type="wicri:Area/PubMed/Corpus">000228</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000228</idno><idno type="wicri:Area/PubMed/Curation">000228</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000228</idno><idno type="wicri:Area/PubMed/Checkpoint">000580</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000580</idno><idno type="wicri:Area/Ncbi/Merge">001376</idno><idno type="wicri:Area/Ncbi/Curation">001376</idno><idno type="wicri:Area/Ncbi/Checkpoint">001376</idno><idno type="wicri:Area/Main/Merge">000625</idno><idno type="wicri:Area/Main/Curation">000623</idno><idno type="wicri:Area/Main/Exploration">000623</idno><idno type="wicri:Area/an2020/Extraction">000623</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Laboratory Diagnosis of Emerging Human Coronavirus Infections - The State of the Art.</title><author><name sortKey="Loeffelholz, Michael J" sort="Loeffelholz, Michael J" uniqKey="Loeffelholz M" first="Michael J" last="Loeffelholz">Michael J. Loeffelholz</name><affiliation wicri:level="1"><nlm:affiliation>Cepheid, Sunnyvale, California, USA, michael.loeffelholz@cepheid.com.</nlm:affiliation><country wicri:rule="url">Oman</country><wicri:regionArea>Cepheid, Sunnyvale, California, USA</wicri:regionArea><wicri:noRegion>USA</wicri:noRegion></affiliation></author><author><name sortKey="Tang, Yi Wei" sort="Tang, Yi Wei" uniqKey="Tang Y" first="Yi-Wei" last="Tang">Yi-Wei Tang</name><affiliation wicri:level="1"><nlm:affiliation>Cepheid, Danaher Diagnostic Platform, Shanghai, China, yi-wei.tang@cepheid.com.</nlm:affiliation><country wicri:rule="url">Oman</country><wicri:regionArea>Cepheid, Danaher Diagnostic Platform, Shanghai, China</wicri:regionArea><wicri:noRegion>China</wicri:noRegion></affiliation></author></analytic><series><title level="j">Emerging microbes & infections</title><idno type="eISSN">2222-1751</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The three unprecedented outbreaks of emerging human coronavirus (HCoV) infections at the beginning of 21st century have highlighted the necessity for readily available, accurate and fast diagnostic testing methods. The laboratory diagnostic methods for human coronavirus infections have evolved substantially, with the development of novel assays as well as the availability of updated tests for emerging ones. Newer laboratory methods are fast, highly sensitive and specific, and are gradually replacing the conventional gold standards. This presentation reviews the current laboratory methods available for testing coronaviruses by focusing on the coronavirus disease 2019 (COVID-19) outbreak going on in Wuhan. Viral pneumonias typically do not result in the production of purulent sputum. Thus, a nasopharyngeal swab is usually the collection method used to obtain a specimen for testing. Nasopharyngeal specimens may miss some infections; a deeper specimen may need to be obtained by bronchoscopy. Alternatively, repeated testing can be used because over time, the likelihood of the SARS-CoV-2 being present in the nasal-pharynx increases. Several integrated, random-access, point-of-care molecular devices are currently under development for fast and accurate diagnosis of SARS-CoV-2 infections. These assays are simple, fast and safe and can be used in the local hospitals and clinics bearing the burden of identifying and treating patients.</div></front></TEI><affiliations><list><country><li>Oman</li></country></list><tree><country name="Oman"><noRegion><name sortKey="Loeffelholz, Michael J" sort="Loeffelholz, Michael J" uniqKey="Loeffelholz M" first="Michael J" last="Loeffelholz">Michael J. Loeffelholz</name></noRegion><name sortKey="Tang, Yi Wei" sort="Tang, Yi Wei" uniqKey="Tang Y" first="Yi-Wei" last="Tang">Yi-Wei Tang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV2/Data/an2020/Analysis
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000623 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/an2020/Analysis/biblio.hfd -nk 000623 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= CovidV2
|flux= an2020
|étape= Analysis
|type= RBID
|clé= pubmed:32196430
|texte= Laboratory Diagnosis of Emerging Human Coronavirus Infections - The State of the Art.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/an2020/Analysis/RBID.i -Sk "pubmed:32196430" \
| HfdSelect -Kh $EXPLOR_AREA/Data/an2020/Analysis/biblio.hfd \
| NlmPubMed2Wicri -a CovidV2
| This area was generated with Dilib version V0.6.33. |  |